2025/01/17 by Akbari, Shabnam, Khalil-Moghaddam, Shiva, Ghobeh, Maryam +2
#Ag Nanoparticle #Antibacterial #Antioxidant #Cytotoxicity #Nanocomposite
paper · doi:10.57647/j.ijnd.2025.1603.20
This study presents a rapid and efficient synthesis of the starch-silver-ascorbic acid nanocomposite (St-Ag-Asc), that starch and ascorbic acid were used as a stabilizer and reducing agent, respectively. The formation of silver nanoparticles was confirmed through spectrophotometry. The bioactivity of the obtained Nanocomposite(NC) was evaluated by testing the antibacterial, antioxidant, and cytotoxicity activity. Antioxidant activity was tested by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric-reducing ability of plasma methods. Based on the results of the DPPH test, NC with a half-maximal inhibitory concentration of 0.035 mg/ml demonstrated the high capability in the scavenging of DPPH free radicals. Additionally, as regards the regeneration of ferric iron, the NC (St-Ag-Asc) with a capability of 0.77 mM iron/mg (NC) revealed good regenerative effects against Asc control. Further, minimum inhibitory concentration (MIC) and the Agar well diffusion methods were employed to determine the antibacterial effects. The NC showed antibacterial effects against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus bacteria with MICs of 0.75 mg/ml, 0.75 mg/ml, and 0.375 mg/ml, respectively. In addition, the cytotoxicity evaluation of the NC by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay confirmed its safety toward normal fibroblast cells, indicating its potential use for advanced biomedical applications.