2024/12/28 by Ishmael Apachigawo, Apachigawo, Ishmael, Dhruvil Solanki +9
Biochemistry, Genetics and Molecular Biology · Medicine · #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Medical Physics (physics.med-ph) #Metabolomics and Mass Spectrometry Studies #Optical Imaging and Spectroscopy Techniques #Optics (physics.optics) #Photodynamic Therapy Research Studies
paper · pdf · doi:10.48550/arxiv.2412.20063
openalex publication_date 2024/12/28 · openalex created_date 2025/01/01 · openalex updated_date 2026/07/28
Photonics/light localization techniques are important in understanding the structural changes in biological tissues at the nano- to sub-micron scale. It is now known that structural alteration starts at the nanoscale at the beginning of cancer progression. This study examines the molecular-specific nano-structural alterations of chronic alcoholism and probiotic effects on colon cancer using a mouse model of colon cancer. Confocal microscopy and mesoscopic light-scattering analysis are applied to quantify structural changes in DNA (chromatin), cytoskeleton, and ki-67 protein cells with appropriate staining dyes. We assessed alcohol-treated and azoxymethane (AOM) with dextran sulfate sodium (DSS)-induced colitis models, including ethanol (EtOH) and probiotic (L.Casei) treatments separately and together. The inverse participation ratio (IPR) technique was employed to quantify the degree of light localization to access the molecular-specific spatial structural disorder as a biomarker for cancer progression detection. Significant enhancement of cancer progression was observed in the alcohol-treated group, and probiotics treatment with alcohol showed partial reversal of these changes in colon cancer. The results underscore the potential of the IPR technique in detecting early structural changes in colon cancer, offering insights into the mitigating effects of probiotics on alcohol-induced enhancement of colon cancer.