Mouse brain and muscle tissues constitutively express high levels of Homer proteins

2000/02/01 by Mikhail M. Soloviev, Mikhail Soloviev, Francisco Ciruela +2 · 22 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Adipose Tissue and Metabolism #Anatomy #Biology #Blot #Cell biology #Gene #Gene expression #Genetics #Messenger RNA #Mitochondrial Function and Pathology #Molecular biology #Muscle Physiology and Disorders #Skeletal muscle #Transcription (linguistics)

paper · pdf · doi:10.1046/j.1432-1327.2000.01078.x

openalex publication_date 2000/02/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/25

Abstract

In order to characterize expression of Homers in mouse brain and peripheral tissues we have developed a coupled reverse transcription (RT)-PCR/restriction digestion approach. This has allowed us to determine the molecular composition and relative levels of the constitutive expression of the Homer-1, -2 and -3 mRNAs across mouse tissues. We report here that mammalian brain constitutively expresses high levels of the Homer-1, -2 and -3 mRNAs. Expression of the Homer-1 mRNAs reaches 66% of the brain total Homer mRNAs expression, followed by Homer-3 mRNA (22%) and Homer-2 mRNAs (12%). Quantitative RT-PCR analysis and the Western blotting using pan-Homer antibody revealed that mouse heart, skeletal muscle and diaphragm constitutively express high levels of the Homer proteins and their mRNAs. We have shown that the molecular profile of expression of Homer-1, -2 and -3 mRNAs in muscle containing tissues resembles that obtained for mammalian brain.

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• Homer proteins in Ca2+ signaling by excitable and non-excitable cells. [europepmc]
• Dynamic regulation of ryanodine receptor type 1 (RyR1) channel activity by Homer 1. [europepmc]
• Homers regulate drug-induced neuroplasticity: implications for addiction. [europepmc]
• Targeting Homer genes using adeno-associated viral vector: lessons learned from behavioural and neurochemical studies. [europepmc]
• Signaling pathways mediating alcohol effects. [europepmc]
• Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission. [europepmc]
• De novo copy number variants are associated with congenital diaphragmatic hernia. [europepmc]
• Cocaine-elicited imbalances in ventromedial prefrontal cortex Homer1 versus Homer2 expression: implications for relapse. [europepmc]
• Expression and function of ryanodine receptor related pathways in PCB tolerant Atlantic killifish (Fundulus heteroclitus) from New Bedford Harbor, MA, USA. [europepmc]
• Homer2 within the nucleus accumbens core bidirectionally regulates alcohol intake by both P and Wistar rats. [europepmc]
• Homer2 within the central nucleus of the amygdala modulates withdrawal-induced anxiety in a mouse model of binge-drinking. [europepmc]
• Behavioral and Neurochemical Phenotyping of Mice Incapable of Homer1a Induction. [europepmc]
• Distinct and Overlapping Expression Patterns of the Homer Family of Scaffolding Proteins and Their Encoding Genes in Developing Murine Cephalic Tissues. [europepmc]
• Transgenic Analyses of Homer2 Function Within Nucleus Accumbens Subregions in the Regulation of Methamphetamine Reward and Reinforcement in Mice. [europepmc]
• Homer1 is a Potential Biomarker for Prognosis in Human Colorectal Carcinoma, Possibly in Association with G3BP1 Signaling. [europepmc]
• Congenital Splay Leg Syndrome in Piglets-Current Knowledge and a New Approach to Etiology. [europepmc]
• Dysregulated Signaling at Postsynaptic Density: A Systematic Review and Translational Appraisal for the Pathophysiology, Clinics, and Antipsychotics' Treatment of Schizophrenia. [europepmc]
• Homer 1 genotype AA variant relates to congenital splay leg syndrome in piglets by repressing Pax7 in myogenic progenitors. [europepmc]
• Increased Homer Activity and NMJ Localization in the Vestibular Lesion het -/- Mouse soleus Muscle. [europepmc]
• Replication and extension of the subregion selectivity of glutamate-related changes within the nucleus accumbens associated with the incubation of cocaine-craving. [europepmc]

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