Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase
1974/09/01 by Stefan L. Marklund, Stefan MARKLUND, Gudrun MARKLUND · 10,321 citations
Chemistry · Environmental Science · #Autoxidation #Biochemistry #Catalase #Catechol #Chemistry #Electrochemical Analysis and Applications #Environmental Toxicology and Ecotoxicology #Enzyme #Free Radicals and Antioxidants #Homolysis #Medicinal chemistry #Nuclear chemistry #Organic chemistry #Oxygen #Photochemistry #Pyrogallol #Radical #Superoxide #Superoxide dismutase
paper · pdf · doi:10.1111/j.1432-1033.1974.tb03714.x
published in European Journal of Biochemistry 47(3), 469-474 (Wiley)
openalex publication_date 1974/09/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Abstract
The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6. The rate of autoxidation increases with increasing pH. At pH 7.9 the reaction is inhibited to 99% by superoxide dismutase, indicating an almost total dependence on the participation of the superoxide anion radical, O 2 · − , in the reaction. Up to pH 9.1 the reaction is still inhibited to over 90% by superoxide dismutase, but at higher alkalinity, O 2 · − ‐independent mechanisms rapidly become dominant. Catalase has no effect on the autoxidation but decreases the oxygen consumption by half, showing that H 2 O 2 is the stable product of oxygen and that H 2 O 2 is not involved in the autoxidation mechanism. A simple and rapid method for the assay of superoxide dismutase is described, based on the ability of the enzyme to inhibit the autoxidation of pyrogallol. A plausible explanation is given for the non‐competitive part of the inhibition of catechol O ‐methyltransferase brought about by pyrogallol.
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