2025/03/28 by Majid Aalizadeh, Shuo Yang, Aalizadeh, Majid +9 · 1 citation
Engineering · Immunology and Microbiology · #Biosensors and Analytical Detection #Biosimilars and Bioanalytical Methods #FOS: Electrical engineering #FOS: Physical sciences #Medical Physics (physics.med-ph) #Microfluidic and Capillary Electrophoresis Applications #Systems and Control (eess.SY) #electronic engineering #information engineering
paper · pdf · doi:10.48550/arxiv.2503.22911
openalex publication_date 2025/03/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
In this work, a miniaturized, automated, and cost-effective ELISA device is designed and implemented, without the utilization of conventional techniques such as pipetting or microfluidic valve technologies. The device has dimensions of 24 cm x 19 cm x 14 cm and weighs <3 Kg. The total hardware cost of the device is estimated to be approximately 1,200, which can be further reduced through optimization during scale-up production. 3D printed disposable parts, including the reagent reservoir disk and the microfluidic connector, have also been developed. IL-6 is used as a model system to demonstrate how the device provides an ELISA measurement. The cost per test is estimated to be less than ten dollars. The compactness, automated operation, along with the cost-effectiveness of this ELISA device, makes it suitable for point-of-care applications in resource-limited regions.