2018/10/07 by Boyi Yang, Yang, Boyi, Nabil Aounallah +1
Biochemistry, Genetics and Molecular Biology · #FOS: Biological sciences #Genomics (q-bio.GN) #Genomics and Chromatin Dynamics #RNA Research and Splicing #RNA modifications and cancer
paper · pdf · doi:10.48550/arxiv.1810.03205
openalex publication_date 2018/10/07 · openalex created_date 2018/10/12 · openalex updated_date 2026/07/28
Transcriptional repressor CTCF is an important regulator of chromatin 3D structure, facilitating the formation of topologically associating domains (TADs). However, its direct effects on gene regulation is less well understood. Here, we utilize previously published ChIP-seq and RNA-seq data to investigate the effects of CTCF on alternative splicing of genes with CTCF sites. We compared the amount of RNA-seq signals in exons upstream and downstream of binding sites following auxin-induced degradation of CTCF in mouse embryonic stem cells. We found that changes in gene expression following CTCF depletion were significant, with a general increase in the presence of upstream exons. We infer that a possible mechanism by which CTCF binding contributes to alternative splicing is by causing pauses in the transcription mechanism during which splicing elements are able to concurrently act on upstream exons already transcribed into RNA.