2017/10/28 by Susannah Waxman, Waxman, Susannah, Ralitsa T. Loewen +10
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Anatomy #Cerebral Venous Sinus Thrombosis #Chemistry #Clearance #FOS: Biological sciences #Geology #Glaucoma #Glaucoma and retinal disorders #Materials science #Medicine #Ophthalmology #Outflow #Retinal Diseases and Treatments #Tissues and Organs (q-bio.TO) #Trabecular meshwork #q-bio.TO
paper · pdf · doi:10.48550/arxiv.1710.10508
published in arXiv (Cornell University) (Cornell University)
arxiv created 2017/10/28 · openalex publication_date 2017/10/28 · arxiv updated 2017/10/31 · openalex created_date 2022/10/04 · openalex updated_date 2026/08/06
Purpose: The rate of conventional aqueous humor outflow is the highest\nnasally. We hypothesized that this is reflected in regionally different outflow\nstructures and analyzed the entire limbus by high-resolution, full-thickness\nribbon-scanning confocal microscopy (RSCM). Methods: We perfused pig eyes by\nanterior chamber cannulation with eight lectin-fluorophore conjugates, followed\nby optical clearance with benzyl alcohol benzyl benzoate (BABB). RSCM and an\nadvanced analysis software (Imaris) were used to reconstruct a\nthree-dimensional, whole-specimen rendering of the perilimbal outflow\nstructures. We performed morphometric analyses of the outflow tract from the\nlevel of the trabecular meshwork (TM) to the scleral vascular plexus (SVP).\nResults: Except for pigmented structures, BABB cleared the entire eye.\nRhodamine-conjugated Glycine max agglutinin (soybean, SBA) labeled the outflow\ntract evenly and retained fluorescence for months. RSCM produced terabyte-sized\nfiles allowing for in silico dissection of outflow tract vessel at a high\nresolution and in 3D. Networks of interconnected lumina were traced from the TM\nto downstream drainage structures. The collector channel (CC) volumes were ten\ntimes smaller than the receiving SVP vessels, the largest of which were in the\ninferior limbus. Proximal CC diameters were up to four times the size of distal\ndiameters and more elliptical at their proximal ends. The largest CCs were\nfound in the superonasal and inferonasal quadrants where the highest outflow\noccurs. Conclusions: RSCM of cleared eyes enabled high-resolution, volumetric\nanalysis of the outflow tract. The proximal structures had greater diameters\nnasally while the SVP was larger in the inferior limbus.\n