2017/03/25 by Nathalie Groß, Dumitru Arian, Groß, Nathalie +5
Biochemistry, Genetics and Molecular Biology · #Biological Physics (physics.bio-ph) #Enzyme Production and Characterization #FOS: Biological sciences #FOS: Physical sciences #Glycosylation and Glycoproteins Research #Protein purification and stability #Proteoglycans and glycosaminoglycans research #Quantitative Methods (q-bio.QM)
paper · pdf · doi:10.48550/arxiv.1703.08663
openalex publication_date 2017/03/25 · openalex created_date 2022/10/01 · openalex updated_date 2026/07/28
Dextran sulfate is semi-synthetic, polydisperse sulfated polysaccharide with\nimportant applications in clinical practice, in the manufacturing of plasma\nderived protein therapeutics and in biomedical research. The sensitive\ndetection of dextran sulfate is relevant to preclinical and clinical drug\ndevelopment projects, the quality control of pharmaceutic formulations, and the\nprocess control in plasma fractionation using dextran sulfate modified\nchromatographic columns. Most analytical methods for the sensitive detection of\ndextran sulfate require multistep protcols and have not been transferred into\ncommercial formats. We describe here the direct quantification of dextran\nsulfate using the commercially available molecular probe Heparin Red, by a\nsimple mix-and-read fluorescence assay. With a sensitive benchtop fluorimeter,\na quantification limit of 310 picogram per mL dextran sulfate is achieved. This\nis superior by at least one order of magnitude to the quantification or\ndetection limit of other reported methods. The outstanding simplicity and\nsensitivity establish Heparin Red as a new analytical tool for the\ndetermination of dextran sulfate.\n