The protein-protein interaction between two spliced leader trans-splicing factors is mediated by two interlinked α-helical domains.

2025/06/18 by Peter Eijlers, Jonathan Pettitt, Berndt Müller
Biochemistry, Genetics and Molecular Biology · #RNA Research and Splicing #Genetics, Aging, and Longevity in Model Organisms #Nuclear Structure and Function

paper · doi:10.17912/micropub.biology.001705

Abstract

. They are components of the SL1 snRNP that donates the spliced leader 1 RNA which replaces the 5' end of most pre-mRNAs. SNA-1 and SNA-2 bind to each other, but the nature of their interaction is unclear. AlphaFold-Multimer predicts that the central region of SNA-1 and the C-terminal region of SNA-2 , each consisting of 3 α-helices, interlink to bring about the interaction between these proteins. Using yeast 2-hybrid assays we demonstrate that these regions are required and sufficient for this unusual mode of interaction between two proteins.

Citations

• UCSF ChimeraX: Tools for structure building and analysis
• Highly accurate protein structure prediction with AlphaFold

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