2025/07/28 by Beatrice M. Bock, Jack Scherer, Faith Parrish +3
Biochemistry, Genetics and Molecular Biology · #Plant tissue culture and regeneration
paper · doi:10.17912/micropub.biology.001772
seeds by submerging them in 95% ethanol for 2 minutes followed by 3.75% sodium hypochlorite for 20 minutes. This approach yielded a low contamination rate (2 out of 95 seeds) and a robust median germination rate (63%). Its simplicity, cost-effectiveness, and accessibility make it a practical option for experiments requiring axenic seeds.