2022/02/22 by Sakshi Singh, Singh, Sakshi, Simon Labouesse +3
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Fluorescence Microscopy Techniques #FOS: Electrical engineering #FOS: Physical sciences #Image and Video Processing (eess.IV) #Optical Coherence Tomography Applications #Optics (physics.optics) #Photoacoustic and Ultrasonic Imaging #electronic engineering #information engineering
paper · pdf · doi:10.48550/arxiv.2202.10644
openalex publication_date 2022/02/22 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent ultrathin endoscopes that can penetrate deep inside the tissue with minimal damage. Here, we present Multiview Scattering Scanning Imaging Confocal (MUSSIC) Microscopy that enables high signal-to-noise ratio (SNR) imaging through a multimode fiber, hence combining the optical sectioning and resolution gain of confocal microscopy with the minimally invasive penetration capability of multimode fibers. The key advance presented here is the high SNR image reconstruction enabled by employing multiple coplanar virtual pinholes to capture multiple perspectives of the object, re-shifting them appropriately and combining them to obtain a high-contrast and high-resolution confocal image. We present the theory for the gain in contrast and resolution in MUSSIC microscopy and validate the concept through experimental results.