2025/04/28 by Alba Muñoz del Río, del Río, Alba Muñoz, Víctor Casero-Alonso +3
Biochemistry, Genetics and Molecular Biology · Chemistry · #Applications (stat.AP) #FOS: Computer and information sciences #Listeria monocytogenes in Food Safety #Microbial Inactivation Methods #thermodynamics and calorimetric analyses
paper · pdf · doi:10.48550/arxiv.2504.19541
openalex publication_date 2025/04/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
This study addresses from the Optimal Experimental Design perspective the use of the isothermal experimentation procedure to precisely estimate the parameters defining models used in predictive microbiology. Starting from a case study set out in the literature, and taking the Baranyi model as the primary model, and the Ratkowsky square-root model as the secondary, D- and c-optimal designs are provided for isothermal experiments, taking the temperature both as a value fixed by the experimenter and as a variable to be designed. The designs calculated show that those commonly used in practice are not efficient enough to estimate the parameters of the secondary model, leading to greater uncertainty in the predictions made via these models. Finally, an analysis is carried out to determine the effect on the efficiency of the possible reduction in the final experimental time.