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Analysis of Transcripts in the Fly Cell Atlas Reveals Additional Cell Populations in the Drosophila melanogaster Ovary

2025/06/27 by Oscar Mendoza Andrade, Zach Wright, Sahel Ghasemzadeh +1
Biochemistry, Genetics and Molecular Biology · #Cell Image Analysis Techniques #Single-cell and spatial transcriptomics

paper · doi:10.17912/micropub.biology.001725

openalex publication_date 2025/06/27 · openalex created_date 2025/08/05 · openalex updated_date 2026/07/01

Abstract

ovary serves as a powerful model system for epithelial morphogenesis. In this study we analyzed previously unidentified ovarian epithelial cells from the Fly Cell Atlas dataset. We identified eight transcriptionally distinct clusters and annotated six of them, including follicle cell developmental stages 9, 10A, and 10B/11. Two additional clusters remain only weakly identified. This work facilitates future use of the ovarian Fly Cell Atlas by providing validated developmental stage markers and filling critical gaps in follicle cell annotation.

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