2025/07/30 by Diana Spencer, Elizabeth Padilla-Crespo, Ek Han Tan
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Plant Pathogens and Resistance #Plant Virus Research Studies #Plant and Fungal Interactions Research
paper · doi:10.17912/micropub.biology.001779
openalex publication_date 2025/07/30 · openalex created_date 2025/10/24 · openalex updated_date 2026/07/01
Standardized protocols for absolute quantification of potato virus Y (PVY) from potato tissue is critical for host-virus dynamic studies. Here, we developed a standardized protocol using a cloned viral sequence as standards to detect and quantify PVY. Starting with total RNA, concentrated via column-based kit, this protocol is able to detect approximately 50 viral copies/reaction from multiple PVY strains. Validation of this protocol confirmed linearity across 8 orders of magnitude with high repeatability, reproducibility and statistical robustness across three independent runs. This protocol offers reliable PVY quantification to manage potato crop health and enables comparative studies with other viral systems.