2025/04/30 by Leila Azimi, Azimi, Leila, Fatemeh Shirkavand +7
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · Medicine · #Bacterial Infections and Vaccines #Haemophilus influenzae #Medicine and health #Neisseria meningitidis #Pneumonia and Respiratory Infections #Streptococcus pneumoniae #primer #sensitivity #vaccines and immunoinformatics approaches
paper · doi:10.3205/dgkh000545
openalex publication_date 2025/04/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/01
Background: using Real-Time PCR technology. Materials and methods: using the Primer Biosoft Allele ID 7.6 application. The study examined the minimum bacterial copy numbers detectable by each primer, as well as their specificity. Results: . The sensitivity and specificity of all primers was 100% (CI: 95%). Conclusion: Using more sensitive primers to detect the bacterial agent responsible for causing bacterial meningitis increases the chance of identifying the causative bacteria. The primers designed in this study could identify the selected bacteria with at least 10 times more sensitivity than the currently available commercial diagnostic kits in Iran.