2013/08/08 by Hunter B. Fraser, Fraser, Hunter B.
Biochemistry, Genetics and Molecular Biology · #CRISPR and Genetic Engineering #DNA Repair Mechanisms #FOS: Biological sciences #Fungal and yeast genetics research #Genomics (q-bio.GN) #Genomics and Chromatin Dynamics #Subcellular Processes (q-bio.SC)
paper · pdf · doi:10.48550/arxiv.1308.1985
openalex publication_date 2013/08/08 · openalex created_date 2022/10/01 · openalex updated_date 2026/07/28
Eukaryotic DNA replication follows a specific temporal program, with some\ngenomic regions consistently replicating earlier than others, yet what\ndetermines this program is largely unknown. Highly transcribed regions have\nbeen observed to replicate in early S-phase in all plant and animal species\nstudied to date, but this relationship is thought to be absent from both\nbudding yeast and fission yeast. No association between cell-cycle regulated\ntranscription and replication timing has been reported for any species. Here I\nshow that in budding yeast, fission yeast, and human, the genes most highly\ntranscribed during S-phase replicate early, whereas those repressed in S-phase\nreplicate late. Transcription during other cell-cycle phases shows either the\nopposite correlation with replication timing, or no relation. The relationship\nis strongest near late-firing origins of replication, which is not consistent\nwith a previously proposed model -- that replication timing may affect\ntranscription -- and instead suggests a potential mechanism involving the\nrecruitment of limiting replication initiation factors during S-phase. These\nresults suggest that S-phase transcription may be an important determinant of\nDNA replication timing across eukaryotes, which may explain the\nwell-established association between transcription and replication timing.\n