2014/11/17 by Johan Zakrisson, Zakrisson, Johan, Krister Wiklund +9
Biochemistry, Genetics and Molecular Biology · Environmental Science · Physics and Astronomy · #Bacteriophages and microbial interactions #Biochemical and Structural Characterization #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Force Microscopy Techniques and Applications
paper · pdf · doi:10.48550/arxiv.1411.4416
openalex publication_date 2014/11/17 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We present a coarse grained rigid multibody model of a subunit assembled helix-like polymer, e.g., adhesion pili expressed by bacteria, that is capable of describing the polymers force-extension response. With building blocks representing individual subunits the model appropriately describes the complex behavior of pili expressed by the gram-negative uropathogenic Escherichia coli bacteria under the action of an external force. Numerical simulations show that the dynamics of the model, which include both the effects of unwinding and rewinding, are in good quantitative agreement with the characteristic force-extension response as observed experimentally for type 1 and P pili. By tuning the model, it is also possible to reproduce the force-extension response in the presence of anti-shaft antibodies, which dramatically changes the mechanical properties. Thus, the model and the results in this work give enhanced understanding of how a pilus unwinds under action of external forces and provide new perspective of the complex bacterial adhesion processes.