A strong H-bond between a cysteine and the catalytic center of a [NiFe]-hydrogenase

2025/07/09 by Karafoulidi-Retsou, Chara, Katz, Sagie, Frielingsdorf, Stefan +3
#500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften #[NiFe]-hydrogenase #cryogenic temperatures #cysteine–metal coordination #infrared spectroscopy #protonation dynamics

paper · doi:10.14279/depositonce-23655

Abstract

Infrared spectroscopy at cryogenic temperatures was used to monitor protonation changes on an H+-accepting, nickel-coordinating active site cysteine of the H2/H+-cycling membrane-bound [NiFe]-hydrogenase from Cupriavidus necator. Surprisingly, we identified another cysteine in the outer coordination sphere forming a strong H-bond with a cysteine thiolate coordinating both nickel and iron of the catalytic center.

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