Estimation of the Concentration of Low-Density Lipoprotein Cholesterol in Plasma, Without Use of the Preparative Ultracentrifuge
1972/06/01 by William T Friedewald, William T. Friedewald, Robert I Levy +3 · 32,564 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Biochemistry #Cancer, Lipids, and Metabolism #Chemistry #Cholesterol #Chromatography #Diabetes, Cardiovascular Risks, and Lipoproteins #Fraction (chemistry) #High-density lipoprotein #Lipoprotein #Lipoproteins and Cardiovascular Health #Medicine #Plasma lipoprotein #Population #Triglyceride #Ultracentrifuge
paper · doi:10.1093/clinchem/18.6.499
published in Clinical Chemistry 18(6), 499-502 (American Association for Clinical Chemistry)
openalex publication_date 1972/06/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
Abstract
Abstract A method for estimating the cholesterol content of the serum low-density lipoprotein fraction (Sf0-20) is presented. The method involves measurements of fasting plasma total cholesterol, triglyceride, and high-density lipoprotein cholesterol concentrations, none of which requires the use of the preparative ultracentrifuge. Comparison of this suggested procedure with the more direct procedure, in which the ultracentrifuge is used, yielded correlation coefficients of .94 to .99, depending on the patient population compared.
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