Calibrating photon counts from a single image

2016/11/17 by Rainer Heintzmann, Peter K. Relich, Heintzmann, Rainer +7 · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Advanced Optical Sensing Technologies #CCD and CMOS Imaging Sensors #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Instrumentation and Methods for Astrophysics (astro-ph.IM)

paper · pdf · doi:10.48550/arxiv.1611.05654

openalex publication_date 2016/11/17 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Current methods for detector gain calibration require acquisition of tens of special calibration images. Here we propose a method that obtains the gain from the actual image for which the photon count is desired by quantifying out-of-band information. We show on simulation and experimental data that our much simpler procedure, which can be retroactively applied to any image, is comparable in precision to traditional gain calibration procedures.

Citations

• Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope

Cited by

• Characterisation of the signal to noise ratio of 2-photon microscopes

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