2014/04/20 by Wolfram Liebermeister, Liebermeister, Wolfram
Biochemistry, Genetics and Molecular Biology · #FOS: Biological sciences #Gene Regulatory Network Analysis #Microbial Metabolic Engineering and Bioproduction #Molecular Networks (q-bio.MN) #Protein Structure and Dynamics
paper · pdf · doi:10.48550/arxiv.1404.5072
openalex publication_date 2014/04/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Metabolic fluxes in cells are governed by physical, physiological, and economic principles. Here I assume an optimal allocation of enzyme resources and postulate a general principle for metabolism: each enzyme must convert less valuable into more valuable metabolites to justify its own cost. The "values", called economic potentials, describe the individual contributions of metabolites to cell fitness. Local value production implies that the cost of an enzyme must be balanced by a benefit, given by the economic potential difference the catalysed reaction multiplied by the flux. Flux profiles that satisfy this principle - i.e. for which consistent potentials can be found - are called economical. Economical fluxes must lead from lower to higher economic potentials, so certain flux cycles are incompatible with any choice of economic potentials and can be excluded. To obtain economical flux profiles, non-beneficial local patterns, called futile motifs, can be systematically removed from a given flux distribution. The principle of local value production resembles thermodynamic principles and complements them in models. Here I describe a modelling framework called Value Balance Analysis (VBA) that uses the two principles and yields the same solution as enzyme cost minimisation (in kinetic models) and flux cost minimisation (in FBA). Given an economical flux distribution, kinetic models in enzyme-optimal states and with these fluxes can be constructed systematically. VBA justifies the principle of minimal fluxes and the exclusion of futile cycles, predicts enzymes that could be plausible targets for regulation, provides criteria for the usage of enzymes and pathways, and explains the choice between high-yield and low-yield flux modes.