Protein Design: From the Aspect of Water Solubility and Stability
2022/08/03 by Rui Qing, Shilei Hao, Eva Smorodina +4 · 32 citations
Biochemistry, Genetics and Molecular Biology · Materials Science · #Protein Structure and Dynamics #Enzyme Structure and Function #RNA and protein synthesis mechanisms
paper · doi:10.1021/acs.chemrev.1c00757
Abstract
transmembrane protein design. Traditional strategies to enhance protein solubility and structural stability are introduced. The designs of stable protein complexes and high-order assemblies are covered. Computational methodologies behind these endeavors, including structure prediction programs, machine learning algorithms, and specialty software dedicated to the evaluation of protein solubility and aggregation, are discussed. The findings and opportunities for Cryo-EM are presented. This review provides an overview of significant progress and prospects in accurate protein design for solubility and stability.
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