Synthesis of 2,6-dihydroxybenzoic acid by decarboxylase-catalyzed carboxylation using CO2 and in situ product removal

2024/01/18 by Ohde, Daniel, Thomas, Benjamin, Bubenheim, Paul +1
#Chemical Engineering #FOS: Chemical engineering

paper · doi:10.15480/882.9058

Abstract

For the enzymatic carboxylation of resorcinol to 2,6-dihydroxybenzoic acid (2,6-DHBA) using gaseous CO2 in an aqueous triethanolamine phase, an adsorption-based in situ product removal was demonstrated. The aim is to improve the reaction yield, which is limited by an unfavourable thermodynamic equilibrium. First, a screening for a high-affinity adsorber was carried out. Then, the application of a suitable adsorber was successfully demonstrated. This enabled achieving reaction yields above 80% using the adsorber for in situ product removal. The applied biotransformation was scaled up to 1.5 L at lab-scale. Furthermore, a downstream process based on the elution and purification of the product bound to the adsorber was developed to obtain 2,6-DHBA in high purity. Recycling is one of the key factors in this system, making it possible to recycle the reaction medium, the adsorber and the solvents in additional batches.

Related

• Enzymatic carboxylation of resorcinol in aqueous triethanolamine at elevated CO2 pressure
• Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase
• Fine bubble-based CO2 capture mediated by triethanolamine coupled to whole cell biotransformation
• Integrated preservation of water activity as key to intensified chemoenzymatic synthesis of bio-based styrene derivatives
• Enabling Heterogeneous Catalysis to Achieve Carbon Neutrality: Directional Catalytic Conversion of CO₂ into Carboxylic Acids