2025/04/30 by Hervé Hugonnet, Hugonnet, Herve, Chulmin Oh +5 · 2 citations
Physics and Astronomy · #Advanced X-ray Imaging Techniques #Digital Holography and Microscopy #FOS: Physical sciences #Optics (physics.optics) #Random lasers and scattering media
paper · pdf · doi:10.48550/arxiv.2504.21369
openalex publication_date 2025/04/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We present the pupil phase series (PPS), a fast and accurate forward scattering algorithm for simulating and inverting multiple light scattering in large biological samples. PPS achieves high-angle scattering accuracy and energy conservation simultaneously by introducing a spatially varying phase modulation in the pupil plane. By expanding the scattering term into a Taylor series, PPS achieves high precision while maintaining computational efficiency. We integrate PPS into a quasi-Newton inverse solver to reconstruct the three-dimensional refractive index of a 180 um-thick human organoid. Compared to linear reconstruction, our method recovers subcellular features-such as nuclei and vesicular structures-deep within the sample volume. PPS offers a scalable and interpretable alternative to conventional solvers, paving the way for high-throughput, label-free imaging of optically thick biological tissues.