2020/04/24 by Emese Tóth, Toth, Emese, Ditta Ungor +13
Biochemistry, Genetics and Molecular Biology · Engineering · Materials Science · #Advanced biosensing and bioanalysis techniques #FOS: Physical sciences #Gold and Silver Nanoparticles Synthesis and Applications #Optics (physics.optics) #Plasmonic and Surface Plasmon Research
paper · pdf · doi:10.48550/arxiv.2004.11671
openalex publication_date 2020/04/24 · openalex created_date 2022/07/26 · openalex updated_date 2026/07/28
Plasmonically enhanced fluorescence is a widely studied and applied\nphenomenon, however only a comparative theoretical and experimental analyses of\ncoupled fluorophores and plasmonic nanoresonators makes it possible to uncover,\nhow this phenomenon can be controlled. A numerical optimization method was\napplied to design configurations that are capable of resulting in an\nenhancement of excitation and emission, moreover of both phenomena\nsimultaneously in coupled Cy5 dye molecule and gold nanorod systems. Parametric\nsensitivity studies revealed, how the fluorescence enhancement depends on the\nmolecule's location, distance and orientation. Coupled systems designed for\nsimultaneous improvement exhibited the highest (intermediate directional) total\nfluorescence enhancement, which is accompanied by intermediate sensitivity to\nthe molecule's parameters, except the location and orientation sensitivity at\nthe excitation wavelength. Gold nanorods with a geometry corresponding to the\npredicted optimal configurations were synthesized, and DNA strands were used to\ncontrol the Cy5 dye molecule distance from the nanorod surface via\nhybridization of the Cy5-labelled oligonucleotide. State-of-the-art dSTORM\nmicroscopy was used to accomplish a proof-of-concept experimental demonstration\nof the theoretically predicted (directional) total fluorescence enhancement.\nThe measured fluorescence enhancement was in good agreement with theoretical\npredictions, thus providing a complete kit to design and prepare coupled\nnanosystems exhibiting plasmonically enhanced fluorescence.\n