2017/04/05 by Luca Quaroni, Katarzyna Pogoda, Quaroni, Luca +5
Biochemistry, Genetics and Molecular Biology · Chemistry · Neuroscience · #Biological Physics (physics.bio-ph) #FOS: Biological sciences #FOS: Physical sciences #Photoreceptor and optogenetics research #Quantitative Methods (q-bio.QM) #Spectroscopy Techniques in Biomedical and Chemical Research #Spectroscopy and Chemometric Analyses #thermodynamics and calorimetric analyses
paper · pdf · doi:10.48550/arxiv.1704.01395
openalex publication_date 2017/04/05 · openalex created_date 2022/10/06 · openalex updated_date 2026/07/28
Atomic Force Microscopy - Infrared (AFM-IR) spectroscopy allows spectroscopic\nstudies in the mid-infrared spectral region with a spatial resolution better\nthan 50 nm. We show that the high spatial resolution can be used to perform\nspectroscopic and imaging studies at the subcellular level in fixed eukaryotic\ncells. We collect AFM-IR images of subcellular structures that include lipid\ndroplets, vesicles and cytoskeletal filaments, by relying on the intrinsic\ncontrast from IR light absorption. We also obtain AFM-IR absorption spectra of\nindividual structures. Most spectra show features that are recognizable in the\nIR absorption spectra of cells and tissue obtained with FTIR technology,\nincluding absorption bands characteristic of phospholipids and polypeptides. We\nalso observe sharp spectral features that we attribute to the nonlinear\nphotothermal response of the system and we propose that they can be used to\nperform sensitive spectroscopy on the nanoscale.\n