Impaired phagocytic mechanism in annexin 1 null macrophages

2006/04/24 by Simon Yona, Sigrid E.M. Heinsbroek, Sigrid E M Heinsbroek +5 · 20 citations
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · Medicine · #S100 Proteins and Annexins #Immune Response and Inflammation #Neonatal Respiratory Health Research

paper · doi:10.1038/sj.bjp.0706730

Abstract

The role of the anti-inflammatory protein annexin-A1 (Anx-A1) in the phagocytic process has been investigated using a murine bone marrow culture-derived macrophage model from Anx-A1(+/+) and Anx-A1(-/-) mice. Macrophages prepared from Anx-A1(-/-) mice exhibited a reduced ingestion of zymosan, Neisseria meningitidis or sheep red blood cells, when compared to Anx-A1(+/+) cells and in the case of zymosan this effect was also mirrored by a reduced clearance in vivo when particles were injected into the peritoneal cavity of Anx-A1(-/-) mice. The ablation of the Anx-A1 gene did not cause any apparent cytoskeletal defects associated with particle ingestion but the cell surface expression of the key adhesion molecule CD11b was depressed in the Anx-A1(-/-) cells providing a possible explanation for the attenuated phagocytic potential of these cells. The production of the cytokines TNFalpha and IL-6 was increased in Anx-A1(-/-) macrophages following phagocytosis of all types of particle.

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