2014/12/15 by Mary Noe, Noe, Mary, Yuval Erez +7
Biochemistry, Genetics and Molecular Biology · Chemistry · Physics and Astronomy · #Advanced biosensing and bioanalysis techniques #Click Chemistry and Applications #DNA and Nucleic Acid Chemistry #cond-mat.soft
paper · pdf · doi:10.48550/arxiv.1412.4562
22 pages, 10 figures, 8 tables
arxiv created 2014/12/15 · arxiv updated 2014/12/16
Steady-state and time-resolved emission techniques were employed to study the photophysical properties of 5-(thien-2-yl)-2'-deoxyuridine (dUTh), an isomorphic fluorescent nucleoside analog. We found that the emission lifetime of dUTh is dependent upon the solvent viscosity and obeys the Förster-Hoffman relation over a wide range of temperatures in 1-propanol, a glass-forming liquid. Upon incorporation into oligodeoxynucleotides, the average emission lifetime significantly increases, and the decay is non-exponential. We use a non-radiative decay model that takes into account the twist angle of the thiophene ring to fit the time-resolved emission decay curves.