Using Fluorescence Recovery After Photobleaching (FRAP) to study\n dynamics of the Structural Maintenance of Chromosome (SMC) complex in vivo

2016/05/25 by Anjana Badrinarayanan, Badrinarayanan, Anjana, Mark C. Leake +1
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #DNA Repair Mechanisms #Bacterial Genetics and Biotechnology

paper · pdf · doi:10.48550/arxiv.1605.07796

Abstract

The SMC complex, MukBEF, is important for chromosome organization and\nsegregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct\nspots (or 'foci') in the cell, where it is thought to carry out most of its\nchromosome associated activities. This chapter outlines the technique of\nFluorescence Recovery After Photobleaching (FRAP) as a method to study the\nproperties of YFP-tagged MukB in fluorescent foci. This method can provide\nimportant insight into the dynamics of MukB on DNA and be used to study its\nbiochemical properties in vivo.\n

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