2018/08/23 by Andrea Cerea, Valeria Caprettini, Cerea, Andrea +17
Biochemistry, Genetics and Molecular Biology · Engineering · Neuroscience · #3D Printing in Biomedical Research #Applied Physics (physics.app-ph) #FOS: Physical sciences #Microfluidic and Bio-sensing Technologies #Neuroscience and Neural Engineering #Pluripotent Stem Cells Research
paper · pdf · doi:10.48550/arxiv.1808.07682
openalex publication_date 2018/08/23 · openalex created_date 2022/08/04 · openalex updated_date 2026/07/28
Biological studies on in vitro cell cultures are of fundamental importance to\ninvestigate cells response to external stimuli, such as new drugs for treatment\nof specific pathologies, or to study communication between electrogenic cells.\nAlthough three-dimensional (3D) nanostructures brought tremendous improvements\non biosensors used for various biological in vitro studies, including drug\ndelivery and electrical recording, there is still a lack of multifunctional\ncapabilities that could help gaining deeper insights in several bio-related\nresearch fields. In this work, the electrical recording of large cell ensembles\nand the intracellular delivery of few selected cells are combined on the same\ndevice by integrating microfluidics channels on the bottom of a multi-electrode\narray decorated with 3D hollow nanostructures. The novel platform allows to\nrecord intracellular-like action potentials from large ensembles of\ncardiomyocytes derived from human Induced Pluripotent Stem Cells (hiPSC) and\nfrom the HL-1 line, while different molecules are selectively delivered into\nsingle/few targeted cells. The proposed approach shows high potential for\nenabling new comprehensive studies that can relate drug effects to network\nlevel cell communication processes.\n