SEBOX Is Essential for Early Embryogenesis at the Two-Cell Stage in the Mouse1

2008/08/28 by Kyeoung-Hwa Kim, Kyeoung‐Hwa Kim, Eun-Young Kim +3 · 8 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #RNA Interference and Gene Delivery #Reproductive Biology and Fertility #Renal and related cancers

paper · doi:10.1095/biolreprod.108.068478

Abstract

Previously, we found high levels of skin-embryo-brain-oocyte homeobox (Sebox) gene expression in germinal vesicle (GV)-stage oocytes. The objective of the present study was to determine the role played by SEBOX in oocyte maturation and early embryogenesis using RNA interference (RNAi). Microinjection of Sebox double-stranded RNA into GV oocytes resulted in a marked decrease in Sebox mRNA and protein expression. However, Sebox RNAi affects neither oocyte maturation rate nor morphological characteristics, including spindle and chromosomal organization of metaphase II oocytes. In addition, Sebox RNAi had no discernible effect on the activities of M-phase promoting factor or mitogen-activated protein kinase. In contrast, microinjection of Sebox double-stranded RNA into pronuclear-stage embryos resulted in holding embryo development at the two-cell (84.9%) and the four- and eight-cell (15.1%) stages. We concluded that Sebox is a new addition to maternal effect genes that produced and stored in oocytes and function in preimplantation embryo development.

Cited by

• Thioredoxin-interacting protein regulates glucose metabolism and affects cytoplasmic streaming in mouse oocytes. [europepmc]
• Associations among Sebox and other MEGs and its effects on early embryogenesis. [europepmc]
• Maternal control of early embryogenesis in mammals. [europepmc]
• The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development. [europepmc]
• Exploratory bioinformatics investigation reveals importance of "junk" DNA in early embryo development. [europepmc]
• Zap70 and downstream RanBP2 are required for the exact timing of the meiotic cell cycle in oocytes. [europepmc]
• Maternal effect factors that contribute to oocytes developmental competence: an update. [europepmc]
• An extended wave of global mRNA deadenylation sets up a switch in translation regulation across the mammalian oocyte-to-embryo transition. [europepmc]

Related

• Dcp1-Bodies in Mouse Oocytes
• The emerging role of the oocyte cortical domain in maturation, fertilization, and development
• The Mammalian Ovary from Genesis to Revelation
• Follicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality
• Protein kinase a modulates Cdc25B activity during meiotic resumption of mouse oocytes