Maturation in Vitro of Pig Oocytes in Protein-Free Culture Media: Fertilization and Subsequent Embryo Development in Vitro1
1998/05/01 by Lalantha R Abeydeera, Lalantha R. Abeydeera, Wei-hua Wang +3 · 20 citations
Medicine · #Reproductive Biology and Fertility #Sperm and Testicular Function #Ovarian function and disorders
paper · pdf · doi:10.1095/biolreprod58.5.1316
Abstract
In the present study, attempts were made to develop a protein-free (PF) in vitro maturation (IVM) system for pig oocytes and to examine subsequent embryo development after in vitro fertilization. In experiment 1, four IVM media were tested: 1) control: North Carolina State University (NCSU) 23+10% porcine follicular fluid; 2) PF-NCSU: NCSU 23+0.1% polyvinyl alcohol (PVA)+1% amino acids; 3) PF-TCM: Tissue culture medium (TCM) 199+PVA; and 4) PF-WM: PF-Waymouth MB 752/1 medium (WM)+PVA. Oocytes were cultured in the respective media containing eCG and hCG (10 IU/ml each) for 20-22 h and then without hormonal supplements for an additional 20-22 h. After culture, the degree of cumulus expansion and frequency of nuclear maturation were determined. Some oocytes were coincubated with frozen-thawed spermatozoa for 5-6 h in modified Tris-buffered medium containing caffeine and BSA. In experiment 2, oocytes were matured in control, PF-TCM, and PF-WM, fertilized in vitro, and cultured for 144 h in NSCU 23+BSA. Fewer (p < 0.01) oocytes reached metaphase II stage in PF-NCSU (45% vs. 80-85%) than in the other media. Oocytes matured in control medium showed the most cumulus expansion, followed by those in PF-TCM and PF-WM; those in PF-NCSU showed very slight expansion. A lower (p < 0.05) penetration rate was obtained for oocytes matured in PF-NCSU than in the control medium (59% vs. 81%). In contrast to those in control (96%) and PF-TCM (93%), oocytes in PF-WM (65%) showed a lower male pronuclear formation. Compared to that in the control, a significantly lower (p < 0.05) cleavage rate was also observed for oocytes matured in PF-WM. Similar proportions of embryos developed to the blastocyst stage when oocytes were matured in control (22%) and PF-TCM (13%). These results indicate that pig oocytes can be successfully matured in a protein-free medium with subsequent development to the blastocyst stage.
Cited by
- Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein-free medium. [europepmc]
- Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos. [europepmc]
- Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine. [europepmc]
- CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation. [europepmc]
- Identification of karyopherin α1 and α7 interacting proteins in porcine tissue. [europepmc]
- Transcriptome Analysis of Pig In Vivo, In Vitro-Fertilized, and Nuclear Transfer Blastocyst-Stage Embryos Treated with Histone Deacetylase Inhibitors Postfusion and Activation Reveals Changes in the Lysosomal Pathway. [europepmc]
- Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo. [europepmc]
- ARID1A, a component of SWI/SNF chromatin remodeling complexes, is required for porcine embryo development. [europepmc]
- Stimulatory Effects of Melatonin on Porcine In Vitro Maturation Are Mediated by MT2 Receptor. [europepmc]
- An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes. [europepmc]
- Ubiquitin A-52 residue ribosomal protein fusion product 1 ( Uba52 ) is essential for preimplantation embryo development. [europepmc]
- Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro. [europepmc]
- Nuclear trafficking dynamics of Bromodomain-containing protein 7 (BRD7), a switch/sucrose non-fermentable (SWI/SNF) chromatin remodelling complex subunit, in porcine oocytes and cleavage-stage embryos. [europepmc]
- NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization†. [europepmc]
- Effects of Porcine Immature Oocyte Vitrification on Actin Microfilament Distribution and Chromatin Integrity During Early Embryo Development in vitro . [europepmc]
- In vitro maturation using αMEM with reduced NaCl enhances maturation and developmental competence of pig oocytes after somatic cell nuclear transfer. [europepmc]
- Inactivation of mammalian spermatozoa on the exposure of TiO 2 nanorods deposited with noble metals. [europepmc]
- The bisphenol S contamination level observed in human follicular fluid affects the development of porcine oocytes. [europepmc]
- Pre-culture with transferrin-Fe 3+ before in vitro maturation improves the developmental competence of porcine oocytes matured in vitro. [europepmc]
- Identification of SWI/SNF Subcomplex GBAF Presence, Intra-Complex Interactions, and Transcriptional Dynamics during Early Porcine Development. [europepmc]
Related