Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes

2019/04/05 by Rudolf K. Thauer · 210 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Environmental Science · #Alkyl #Anaerobic oxidation of methane #Chemistry #Cofactor #Enzyme #Inorganic chemistry #Medicinal chemistry #Methane #Methane Hydrates and Related Phenomena #Methanogenesis #Methyl group #Microbial metabolism and enzyme function #Nickel #Organic chemistry #Porphyrin Metabolism and Disorders #Redox #Reductase #Stereochemistry

paper · pdf · doi:10.1021/acs.biochem.9b00164

published in Biochemistry 58(52), 5198-5220 (American Chemical Society)

openalex publication_date 2019/04/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04

Abstract

' = -140 mV) gets too high. Methanogens therefore harbor an enzyme system for the reactivation of inactivated MCR in an ATP-dependent reduction reaction. Purification of active MCR in the Ni(I) oxidation state is very challenging and has been achieved in only a few laboratories. This perspective reviews the function, structure, and properties of MCR, what is known and not known about the catalytic mechanism, how the inactive enzyme is reactivated, and what remains to be discovered.

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