Excitation energy transfer in DNA: Duplex melting and transfer from normal bases to 2-aminopurine

1993/11/01 by Thomas M. Nordlund, Daguang Xu, Kervin O. Evans · 6 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Physics and Astronomy · #Biology #Chemistry #Citation #Computer science #DNA #DNA and Nucleic Acid Chemistry #Duplex (building) #Ecology #Information retrieval #Information transfer #Library science #Photochemistry and Electron Transfer Studies #Reuse #Social media #Spectroscopy and Quantum Chemical Studies #Telecommunications #Transfer (computing) #World Wide Web

paper · doi:10.1021/bi00096a020

openalex publication_date 1993/11/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/11

Abstract

Absorption and fluorescence excitation and emission spectra of the B DNA duplex decamer d[CTGA(2AP)TTCAG]2, where emission from the 2AP (2-aminopurine) base dominates, have been measured as a function of temperature. A low-temperature excitation band in the 260-270-nm region disappears near the duplex melting temperature, Tm = 27 degrees C, but then reappears at higher temperatures. Singlet-singlet energy transfer thus occurs between the normal DNA bases and the 2AP base in the B-helical conformation and to a lesser extent in the structurally-mobile melted conformation. The measured efficiency of transfer is 4-5% at 4 degrees C, near 0 at 30 degrees C, and rises again to 1% at 48 degrees C. Nearest-neighbor-only singlet transfer is likely. Such transfer does not offer a likely explanation for UV damage distributions in DNA.

Cited by

• Distinguishing "looped-out" and "stacked-in" DNA bulge conformation using fluorescent 2-aminopurine replacing a purine base. [europepmc]
• Fluorescence probing of aminofluorene-induced conformational heterogeneity in DNA duplexes. [europepmc]
• Fluorescent analogs of biomolecular building blocks: design, properties, and applications. [europepmc]
• Exciplexes and conical intersections lead to fluorescence quenching in π-stacked dimers of 2-aminopurine with natural purine nucleobases. [europepmc]
• High-Throughput Exonuclease Assay Based on the Fluorescent Base Analogue 2-Aminopurine. [europepmc]
• New insights into the structure and dynamics of the epigenetic modifications on DNA. [europepmc]

Related

• Luminescence of Nucleosides, Nucleotides, and DNA at 77°K
• Fifty years of DNA “Breathing”: Reflections on old and new approaches
• Mutagenesis and Metallic DNA
• The Hidden Routes of DNA Photostability: Charge and Proton Transfer in Excited Cytosine–Guanine Tetramers
• Duplex-single strand denaturing transition in DNA oligomers