2024/01/10 by Tibor Novák, Novák, T., Péter Bíró +13
Engineering · Materials Science · #FOS: Physical sciences #Gold and Silver Nanoparticles Synthesis and Applications #Near-Field Optical Microscopy #Optics (physics.optics) #Plasmonic and Surface Plasmon Research
paper · pdf · doi:10.48550/arxiv.2401.05231
openalex publication_date 2024/01/10 · openalex created_date 2024/10/10 · openalex updated_date 2026/07/28
Single molecule microscopy has proven to be an effective tool to characterize the fluorophore-plasmonic structure interaction. However, as specific information is hidden in the emission, sophisticated evaluation is required. Here we investigated the emission polarization of rotationally mobile fluorophores near plasmonic Au/Ag alloy nanospheres both theoretically and experimentally. Our work surpasses the results of the previous studies in that it considers the rotational mobility of the fluorophores attached to metallic nanoparticles. Through theoretical modeling and elaborate numerical calculations we determined the expected measurable fluorescence polarization, and via DNA-PAINT single molecule measurements we validated the theoretical predictions. Our findings suggest that through measuring the polarization state of a sequence of single-molecule events, it is possible to infer the interaction strength of the dye-nanosphere system. A precise description requires elaborate calculations, however, a simplified model is able to predict the tendency of the polarization state with the change of experimental parameters.