Myomir dysregulation and reactive oxygen species in aged human satellite cells

2016/03/11 by Ester Sara Di Filippo, Rosa Mancinelli, Tiziana Pietrangelo +4 · 21 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Muscle Physiology and Disorders #Telomeres, Telomerase, and Senescence #Nutrition and Health in Aging

paper · doi:10.1016/j.bbrc.2016.03.030

Abstract

Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.

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