Phospholipid-driven differences determine the action of the synthetic antimicrobial peptide OP-145 on Gram-positive bacterial and mammalian membrane model systems

2015/07/23 by Nermina Malanovic, Nermina Malanović, Regina Leber +8 · 81 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Immunology and Microbiology · #Antimicrobial Peptides and Activities #Antimicrobial peptides #Bilayer #Biochemical and Structural Characterization #Biochemistry #Biology #Biophysics #Cathelicidin #Cell wall #Chemistry #Circular dichroism #Lipid Membrane Structure and Behavior #Lipid bilayer #Liposome #Magainin #Melittin #Membrane #Peptide #Peptidoglycan #Phosphatidylcholine #Phosphatidylglycerol #Phospholipid

paper · pdf · doi:10.1016/j.bbamem.2015.07.010

published in Biochimica et Biophysica Acta (BBA) - Biomembranes 1848(10), 2437-2447 (Elsevier BV)

openalex publication_date 2015/07/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/02

Abstract

OP-145, a synthetic antimicrobial peptide developed from a screen of the human cathelicidin LL-37, displays strong antibacterial activities and is--at considerably higher concentrations--lytic to human cells. To obtain more insight into its actions, we investigated the interactions between OP-145 and liposomes composed of phosphatidylglycerol (PG) and phosphatidylcholine (PC), resembling bacterial and mammalian membranes, respectively. Circular dichroism analyses of OP-145 demonstrated a predominant α-helical conformation in the presence of both membrane mimics, indicating that the different membrane-perturbation mechanisms are not due to different secondary structures. Membrane thinning and formation of quasi-interdigitated lipid-peptide structures was observed in PG bilayers, while OP-145 led to disintegration of PC liposomes into disk-like micelles and bilayer sheets. Although OP-145 was capable of binding lipoteichoic acid and peptidoglycan, the presence of these bacterial cell wall components did not retain OP-145 and hence did not interfere with the activity of the peptide toward PG membranes. Furthermore, physiological Ca++ concentrations did neither influence the membrane activity of OP-145 in model systems nor the killing of Staphylococcus aureus. However, addition of OP-145 at physiological Ca++-concentrations to PG membranes, but not PC membranes, resulted in the formation of elongated enrolled structures similar to cochleate-like structures. In summary, phospholipid-driven differences in incorporation of OP-145 into the lipid bilayers govern the membrane activity of the peptide on bacterial and mammalian membrane mimics.

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