2023/05/20 by Jingyi Wang, You Wu, Wang, Jingyi +13
Engineering · Physics and Astronomy · #Digital Holography and Microscopy #FOS: Physical sciences #Medical Physics (physics.med-ph) #Microfluidic and Bio-sensing Technologies #Near-Field Optical Microscopy #Optics (physics.optics)
paper · pdf · doi:10.48550/arxiv.2305.12153
openalex publication_date 2023/05/20 · openalex created_date 2023/05/24 · openalex updated_date 2026/07/28
The flexible endoscope is a minimally invasive tool in clinical settings, but most of them rely on exogenous staining for diagnosis to provide qualitative information. Here, we demonstrated a flexible endoscopic microscopy (FEM) with diffracted gradient light for quantitative phase imaging of unlabeled thick samples. Our instrument features a small form factor fiber bundle as the endoscope probe, cellular-level lateral and axial resolutions, and direct phase measurement via simple field modulation. By testing pathologic slices, thick opaque mammalian tissue ex vivo and wound healing in vivo, FEM identifies normal and tumor glandular structures, secreta, and tomographic skin layers. With the advantages of direct morphological and phase measurement, high resolution, and thin fiber tip, the label-free FEM could be an attractive tool for various clinical applications.