2009/12/24 by Vasil G. Bregadze, Bregadze, Vasil G., Irina G. Khutsishvili +5
Biochemistry, Genetics and Molecular Biology · Chemistry · #Biological Physics (physics.bio-ph) #DNA and Nucleic Acid Chemistry #FOS: Physical sciences #History and advancements in chemistry #Various Chemistry Research Topics
paper · pdf · doi:10.48550/arxiv.0912.4866
openalex publication_date 2009/12/24 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/28
The goal of the work was to study the role of GC alternative dimmers in the binding of DNA with Ni (II) ions. The method of ultraviolet difference spectroscopy has been applied to investigate Ni (II) ions interactions with DNA extracted from Clostridium perfringens, Mice liver (C3HA line), Calf thymus, Salmon sperm, Herring sperm, E.coli, Micrococcus luteus and polynucleotides Poly (dA-dT)xPoly (dA-dT), Poly (dG)x Poly (dC), Poly (dG-dC)xPoly (dG-dC). It is shown that Ni (II) ions at outer-spherical binding with DNA double helix from the side of the major groove choose more stable dimmers 3^'-C-G-5^' . . 5^'-G-C-3^' and get bound with N7 atoms of both guanines in dimmer forming G-G interstrand crosslink. It directly correlates to the process of forming point defects of Watson-Crick wrong pair type (creation of rare keto-enolic and amino-imino tautomeric forms) and depurinization.