2009/03/16 by Alexander Heifetz, Heifetz, Alexander, Alexander Z. Patashinski +3
Engineering · Medicine · #Advanced Image Fusion Techniques #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Optical Imaging and Spectroscopy Techniques #Optics (physics.optics) #Radiative Heat Transfer Studies
paper · pdf · doi:10.48550/arxiv.0903.2832
openalex publication_date 2009/03/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Possible origins of the experimentally observed beat-like signal in elastic light backscattering from an epithelial biological cell are identified. The source of the beat-like signal is most likely the cellular nucleus, which is a spheroidal particle with an optically sharp edge, low relative index of refraction, and a size parameter in the resonant range; another possible source is an agglomeration of cell organelles of a similar size. Using Mie theory computer simulations for a dielectric sphere with biological nucleus-like parameters, we have shown that backscattering as a function of size parameter has a beat-like pattern. The high frequency periodic oscillation is the single scattering contribution, while multiple scattering events give slowly varying oscillatory contributions. With increasing contrast, the backscattering signal changes from a pure sinusoid to that of a beat signal. The beat-like pattern in scattering from a low contrast particle does not depend on any particular features of the sphere; a similar pattern is observable in scattering from star-like non-spherical objects with a sharp optical contrast and smooth shape; in this case, the high frequency of oscillations depends on the object orientation relative to incident light