An AI-directed analytical study on the optical transmission microscopic images of Pseudomonas aeruginosa in planktonic and biofilm states

2024/12/24 by Bidisha Sengupta, Mousa Alrubayan, Sengupta, Bidisha +13
Biochemistry, Genetics and Molecular Biology · Engineering · #Bacterial biofilms and quorum sensing #Cell Image Analysis Techniques #Biosensors and Analytical Detection

paper · doi:10.48550/arxiv.2412.18205

Abstract

with high accuracy. Aptamer DNA templated silver nanocluster (Ag-NC) was used to prevent biofilm formation, which produced images of the planktonic states of the bacteria. Large-volume bright field images of bacterial biofilms were used to design the AI model. In particular, we used U-Net with ResNet encoder enhancement to segment biofilm images for AI analysis. Different degrees of biofilm structures can be efficiently detected using ResNet18 and ResNet34 backbones. The potential applications of this technique are also discussed.

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