The High-Level Expression in Escherichia coli of the Membrane-Bound Form of Human and Rat Cytochrome b5 and Studies on Their Mechanism of Function

1994/08/01 by P L Holmans, P.L. Holmans, Manjunath S. Shet +5 · 16 citations
Pharmacology, Toxicology and Pharmaceutics · Biochemistry, Genetics and Molecular Biology · Environmental Science · #Pharmacogenetics and Drug Metabolism #Receptor Mechanisms and Signaling #Effects and risks of endocrine disrupting chemicals

paper · doi:10.1006/abbi.1994.1345

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• A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. [europepmc]
• Role of NADPH-cytochrome P450 reductase and cytochrome-b5/NADH-b5 reductase in variability of CYP3A activity in human liver microsomes. [europepmc]
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• Cerivastatin in vitro metabolism by CYP2C8 variants found in patients experiencing rhabdomyolysis. [europepmc]
• Drug metabolism by CYP2C8.3 is determined by substrate dependent interactions with cytochrome P450 reductase and cytochrome b5. [europepmc]
• Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme. [europepmc]
• Cross-linking mass spectrometry and mutagenesis confirm the functional importance of surface interactions between CYP3A4 and holo/apo cytochrome b(5). [europepmc]
• Functional characterization of cytochromes P450 2B from the desert woodrat Neotoma lepida. [europepmc]
• Functional importance of a peripheral pocket in mammalian cytochrome P450 2B enzymes. [europepmc]
• Mechanistic Scrutiny Identifies a Kinetic Role for Cytochrome b5 Regulation of Human Cytochrome P450c17 (CYP17A1, P450 17A1). [europepmc]
• Coumarin Derivatives as Substrate Probes of Mammalian Cytochromes P450 2B4 and 2B6: Assessing the Importance of 7-Alkoxy Chain Length, Halogen Substitution, and Non-Active Site Mutations. [europepmc]
• Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis. [europepmc]
• Use of Phenoxyaniline Analogues To Generate Biochemical Insights into the Interactio n of Polybrominated Diphenyl Ether with CYP2B Enzymes. [europepmc]
• Bielectrode Strategy for Determination of CYP2E1 Catalytic Activity: Electrodes with Bactosomes and Voltammetric Determination of 6-Hydroxychlorzoxazone. [europepmc]
• Cytochrome b5 occurrence in giant and other viruses belonging to the phylum Nucleocytoviricota. [europepmc]