Arbitrary multisite two-photon excitation in four dimensions

2009/08/31 by Vincent R. Daria, Vincent Ricardo Daria, Christian Stricker +4 · 9 citations
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Nonlinear Optical Materials Studies #Force Microscopy Techniques and Applications

paper · pdf · doi:10.1063/1.3216581

Abstract

We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.

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• Imaging membrane potential changes from dendritic spines using computer-generated holography. [europepmc]
• A compact holographic projector module for high-resolution 3D multi-site two-photon photostimulation. [europepmc]
• Real Time Generation of Three Dimensional Patterns for Multiphoton Stimulation. [europepmc]
• Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond. [europepmc]
• Red-shifted excitation enhances the sensitivity of red genetically encoded Ca 2+ indicator and enables crosstalk-free two-photon holographic optophysiology. [europepmc]

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