2025/10/22 by Javadpour, Sadaf, Ehsani, Mohammadhossein, Houshmand, Elahe +3
#Alginate #Biomimetic bone regeneration #Bone dressing #Carboxymethyl cellulose #Tricalcium Phosphate
paper · doi:10.57647/pibm.2023.122318
After tooth extraction, bone loss is still a major clinical concern, especially for operations that call for the implantation of an implant later on. Promising materials for scaffold construction in bone tissue engineering include β-tricalcium phosphate (TCP), a resorbable ceramic with osteoconductive qualities, and chitosan, a natural polymer with exceptional biocompatibility. The purpose of this work was to create and assess composite chitosan sponges with varying TCP concentrations for possible application as extraction socket preservation (ESP) materials. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) were used to analyze chitosan sponges that had been produced with 1%, 3%, and 5% (w/v) TCP. Degradation rate, blood clotting capacity, porosity, and water absorption were assessed. Biocompatibility and mineralization were evaluated using MTT and Alizarin Red staining assays on MG63 cells. FTIR and XRD showed increased molecular interaction and crystallinity, especially in Cs/T3. The Cs/T5 sponge showed the highest blood absorption (91% RBC) and significantly enhanced cell viability and mineralization in MG63 cells. Water absorption remained consistently high (~97%) across all samples. Cs/TCP composite scaffolds demonstrated favorable physicochemical and biological properties, with Cs/T5 showing particular promise due to its superior cellular response and hemostatic activity. These findings support further exploration of Cs/TCP sponges for bone regeneration and dental socket preservation applications.