2024/06/02 by Lulu Xu, Peiwu Qin, Xu, Lulu +5
Biochemistry, Genetics and Molecular Biology · Medicine · Neuroscience · #Adipokines, Inflammation, and Metabolic Diseases #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Neuroinflammation and Neurodegeneration Mechanisms #Zebrafish Biomedical Research Applications
paper · pdf · doi:10.48550/arxiv.2406.00603
openalex publication_date 2024/06/02 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
In the field of environmental toxicology, rapid and precise assessment of the inflammatory response to pollutants in biological models is critical. This study leverages the power of deep learning to enable automated assessments of zebrafish, a model organism widely used for its translational relevance to human disease pathways. We present an innovative approach to assessing inflammatory responses in zebrafish exposed to various pollutants through an end-to-end deep learning model. The model employs a Unet-based architecture to automatically process high-throughput lateral zebrafish images, segmenting specific regions and quantifying neutrophils as inflammation markers. Alongside imaging, qPCR analysis offers gene expression insights, revealing the molecular impact of exposure on inflammatory pathways. Moreover, the deep learning model was packaged as a user-friendly executable file (.exe), facilitating widespread application by enabling use on virtually any computer without the need for specialized software or training.