2014/01/01 by Philippe Barré, Barre, Philippe, Gaelle Chupeau +27
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Forage grasses #Genetic Mapping and Diversity in Plants and Animals #Genetic and phenotypic traits in livestock #Graminée fourragère #Lolium perenne L #Lolium pérenne #Marqueur moléculaire #Molecular markers #Perennial ryegrass #Plant Taxonomy and Phylogenetics #Polymorphisme nucléotidique simple SNP #Qta #SNP #Sélection assistée par marqueurs
paper · doi:10.17180/a1hd-dt51
openalex publication_date 2014/01/01 · openalex created_date 2017/07/21 · openalex updated_date 2026/07/25
Forage varieties are synthetic varieties obtained by the intercross of a small number of elite genotypes selected in recurrent selection programs. This breeding method has been successful to improve forage species but its efficiency could be increased by the use of molecular markers. The goal of this study was to develop a tool for an easy genotyping of thousands of individuals with an hundred markers evenly spread across the genome in perennial ryegrass. The strategy was to design primer pairs in conserved regions on both sides of an intron for amplification and sequencing in genotypes of interest (polycross parents). Sequences were used to develop SNP markers. We developed 363 primer pairs evenly distributed across the genome with good amplification in perennial ryegrass. Moreover, these primer pairs showed an excellent transferability to other forage grass species (between 73 and 97% in fescues and 77% in cocksfoot). Polymorphism study on seven perennial ryegrass genotypes revealed one SNP every 42 bases on average. Only 38% of the SNPs were heterozygous in more than one genotype. This required to develop specific SNP markers in order to study segregating progenies within each of the seven genotypes.