2025/04/30 by J. N. Brasil, E. S. Jereissati, Brasil, J. N. +5
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · #Botanical Research and Applications #Chemical synthesis and alkaloids #Plant tissue culture and regeneration
paper · doi:10.5073/jabfq.2005.079.027
openalex publication_date 2025/04/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Here we present a protocol for the mass propagation of Nopalea cochenillifera, a cactus species widely used in semi-arid regions as a forage crop. Arcoles were isolated from young cladodes of fieldgrown plants and cultivated in Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) at 7.5 µM and indolacetic acid (IAA) at 1.0 µM. Axillary bud proliferation was achieved by transferring in vitro grown shoots to hormone-free MS medium. The Plantlets successfully survived acclimatization ex vitro by using the protocol presented here. it is possible to reach a propagation rate of up 20 individuals for each cycle of multiplication.