Alkaline Phosphatase-Instructed Self-Assembly of Gadolinium Nanofibers for Enhanced T₂-Weighted Magnetic Resonance Imaging of Tumor

2017/06/19 by Ling Dong, Junchao Qian, Zijuan Hai +4 · 14 citations
Materials Science · Medicine · Chemistry · #Lanthanide and Transition Metal Complexes #Advanced MRI Techniques and Applications #Advanced NMR Techniques and Applications

paper · pdf · doi:10.1021/acs.analchem.7b00621

Abstract

High Resolution Image Download MS PowerPoint Slide Alkaline phosphatase (ALP) is an important enzyme but using ALP-instructed self-assembly of gadolinium nanofibers for enhanced T 2 -weighted magnetic resonance imaging (MRI) of tumor has not been reported. In this work, we rationally designed a hydrogelator Nap-FFFYp-EDA-DOTA(Gd) ( 1P ) which, under the catalysis of ALP, was able to self-assemble into gadolinium nanofibers to form hydrogel Gel I for enhanced T 2 -weighted MR imaging of ALP activity in vitro and in tumor. T 2 phantom MR imaging indicated that the transverse relaxivity ( r 2 ) value of Gel I was 33.9% higher than that of 1P and both of them were 1 order of magnitude higher than that of Gd-DTPA. In vivo T 2 -weighted MR imaging showed that, at 9.4 T, ALP-overexpressing HeLa tumors of 1P -injected mice showed obviously enhanced T 2 contrast. We anticipate that, by replacing ALP with other enzymes, our approach could be applied for MR diagnosis of other diseases in the future.

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