Spontaneous α-N-6-Phosphogluconoylation of a “His Tag” inEscherichia coli:The Cause of Extra Mass of 258 or 178 Da in Fusion Proteins

1999/02/01 by Kieran F. Geoghegan, Henry B.F. Dixon, H. B. F. Dixon +12 · 27 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Materials Science · #Acylation #Amino Acid Enzymes and Metabolism #Biochemical and Molecular Research #Biochemistry #Chemistry #Dephosphorylation #Enzyme #Enzyme Structure and Function #Phosphatase

paper · doi:10.1006/abio.1998.2990

openalex publication_date 1999/02/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/25

Cited by

• Structural Heterogeneity of Proteoform-Ligand Complexes in Adenosine Monophosphate-Activated Protein Kinase Uncovered by Integrated Top-Down Mass Spectrometry
• Discovery and biosynthetic pathway analysis of cyclopentane–β-lactone globilactone A
• In vivo and in vitro trans-acylation by BryP, the putative bryostatin pathway acyltransferase derived from an uncultured marine symbiont. [europepmc]
• Characterization of unusual families of ATG8-like proteins and ATG12 in the protozoan parasite Leishmania major. [europepmc]
• Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism. [europepmc]
• Developing dual and specific inhibitors of dimethylarginine dimethylaminohydrolase-1 and nitric oxide synthase: toward a targeted polypharmacology to control nitric oxide. [europepmc]
• The biochemical basis for stereochemical control in polyketide biosynthesis. [europepmc]
• Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli. [europepmc]
• Crystal structure of reduced and of oxidized peroxiredoxin IV enzyme reveals a stable oxidized decamer and a non-disulfide-bonded intermediate in the catalytic cycle. [europepmc]
• Demonstration of starter unit interprotein transfer from a fatty acid synthase to a multidomain, nonreducing polyketide synthase. [europepmc]
• Pseudouridine monophosphate glycosidase: a new glycosidase mechanism. [europepmc]
• Regioselective covalent immobilization of recombinant antibody-binding proteins A, G, and L for construction of antibody arrays. [europepmc]
• Calcium-dependent stoichiometries of the KCa2.2 (SK) intracellular domain/calmodulin complex in solution. [europepmc]
• Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis. [europepmc]
• Crystallographic and spectroscopic snapshots reveal a dehydrogenase in action. [europepmc]
• Structural Characterization and Bioactivity Analysis of the Two-Component Lantibiotic Flv System from a Ruminant Bacterium. [europepmc]
• S-Nitrosation of Conserved Cysteines Modulates Activity and Stability of S-Nitrosoglutathione Reductase (GSNOR). [europepmc]
• An histidine covalent receptor and butenolide complex mediates strigolactone perception. [europepmc]
• Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. [europepmc]
• Multivalent Small-Molecule Pan-RAS Inhibitors. [europepmc]
• Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex. [europepmc]
• Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF 165 Neutralization. [europepmc]
• Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence. [europepmc]
• Molecular analysis of human Ero1 reveals novel regulatory mechanisms for oxidative protein folding. [europepmc]
• Structural and biochemical studies of the glucuronoyl esterase Ot CE15A illuminate its interaction with lignocellulosic components. [europepmc]
• Use of an asparaginyl endopeptidase for chemo-enzymatic peptide and protein labeling. [europepmc]
• Exploiting Protein N-Terminus for Site-Specific Bioconjugation. [europepmc]