Assessment of human plasma and urine sample preparation for reproducible and high-throughput UHPLC-MS clinical metabolic phenotyping

2020/01/01 by Andrew D. Southam, Liam D. Haglington, Lukáš Najdekr +3 · 23 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · #Metabolomics and Mass Spectrometry Studies #Gut microbiota and health #Analytical Chemistry and Chromatography

paper · pdf · doi:10.1039/d0an01319f

Abstract

UHPLC-MS plasma lipidomics, monophasic 100% isopropanol had the highest detection response of all annotated lipid classes with high reproducibility. Increasing monophasic incubation time and temperature had little benefit on metabolite and lipid yield and reproducibility for all methods.

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